Wuhan EnkiLife Science&Technology Co., Ltd.

Detection Principle:
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.

Detection Method: Fluorometric method, Flow cytometry
Sample Type: Cell samples
Assay Time: 40 min
Detection Instrument: Flow Cytometer
Dye Type: FITC
Ex/Em: 490/530
Channel Set: FITC
Other Reagents Required: PBS, Deionized water
Storage: This product can be stored at 2-8°C for 12 months with shading light.
Expiration date: 12 months
Shipping: Ice bag