Wuhan EnkiLife Science&Technology Co., Ltd.

Test Principle：

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.

Key Features:

Full Name: Interleukin 6
Synonym: IL6; BSF2; HGF; HSF; IFNB2; CDF
Assay Type: Sandwich
Reactivity: Human
Range: 1.56-100pg/mL
Sensitivity: 0.93pg/mL
Sample Type: serum, plasma and other biological fluids
Sample volume: 100μL
UNIProt ID: P05231
Microplate: 96-wells plate breakable into 12 x 8 wells strip

Storage：

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.